u266 b1 human multiple myeloma b lymphocyte cell line  (ATCC)

Journal: Nutrients

Article Title: Immunomodulatory Effects of Lactobacillus brevis NES-428 in a Hyperthyroidism Mouse Model: Potential Applications for Graves’ Disease

doi: 10.3390/nu17182967

Figure Lengend Snippet: NES-428 modulates pro- and anti-inflammatory cytokine transcription in Jurkat T cells. In the non-treated (NC) group, the cells were simply maintained for 24 h without any stimulation. In the stimulated control (ST) group, the same number of cells was exposed to 50 ng/mL of phorbol 12-myristate 13-acetate (PMA) and 1 µg/mL ionomycin for 6 h. For the NES-428 group, Jurkat cells were first incubated for 24 h with heat-killed NES-428, after which they were stimulated by 50 ng/mL PMA and 1 µg/mL ionomycin for 6 h. Total RNA was extracted from each group, and cytokine expression was analyzed by quantitative real-time PCR. Relative mRNA expression of IL-2, IL-4, IL-6, IL-12, TNF-α and IFN-γ was shown. NC = untreated control; ST = stimulated positive control. Data represent mean ± SD of three independent experiments; p < 0.05 vs. NC.

Article Snippet: To investigate the immunomodulatory effects of NES-428, the human T lymphocyte cell line Jurkat (ATCC TIB-152) was employed for cytokine expression analysis.

Techniques: Control, Incubation, Expressing, Real-time Polymerase Chain Reaction, Positive Control